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KMID : 0616120120430040328
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Korean Journal of Pharmacognosy
2012 Volume.43 No. 4 p.328 ~ p.337
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Comparative Study on Biological Activities and Ingredient Contents of Different Solvent Extracts of Samchulkunbi-tang
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Jin Seong-Eun
Seo Chang-Seob
Ha Hye-Kyung
Lim Hye-Sun
Kim Ye-Ji
Jeon Woo-Young
Yoo Sae-Room
Kim Jung-Hoon
Shin In-Sik
Kim Seong-Sil
Shin Na-Ra
Lee Mee-Young
Jeong Soo-Jin
Kim Ohn-Soon
Shin Hyeun-Kyoo
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Abstract
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In order to investigate anti-inflammatory, anti-allergic and anti-obesity activities of Samchulkunbi-tang (SCT; Shen zhu jian pi-tang) water and 70% ethanol (EtOH) extracts, in vitro inhibitory activities against nitric oxide (NO), prostaglandin E2 (PGE2), interleukin (IL)-6 and tumor necrosis factor (TNF)-α production in lipopolysaccharide-stimulated RAW 264.7 cells, and macrophage-derived chemokine (MDC/CCL22) and regulated on activation of normal T-cell-expressed and -secreted
(RANTES/CCL5) production in TNF-α/interferon-γ-stimulated HaCaT and BEAS-2B cells as well as glycerol-3-phosphate
dehydrogenase (GPDH) activity and leptin production in 3T3-L1 cells were determined. A HPLC was used for quantification
of the seven marker components (albiflorin, paeoniflorin, liquiritin, naringin, hesperidin, poncirin and glycyrrhizin) of SCT
water and 70% EtOH extracts. SCT showed inhibitory effects against MDC and RANTES production in HaCaT cells, as well
as RANTES production in BEAS-2B cells. In addition, SCT reduced not only NO, PGE2, IL-6 and TNF-α production in RAW
264.7 cells, but also GPDH activity and leptin production in 3T3-L1 cells. Furthermore, the biological activities and the contents of six compounds (except paeoniflorin) were higher in 70% EtOH extract than water extract. These results suggest that SCT has anti-inflammatory, anti-allergic and anti-obesity activities. These efficacies of 70% EtOH extract are relatively higher than that of water extract.
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KEYWORD
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Samchulkunbi-tang, Different solvent extract, Anti-inflammation, Anti-allergy, Anti-obesity
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